Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays

Samadhan J Jadhao; Larry J Anderson

doi:10.1007/978-1-4939-3687-8_4

Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays

Methods Mol Biol. 2016:1442:41-52. doi: 10.1007/978-1-4939-3687-8_4.

Authors

Samadhan J Jadhao¹, Larry J Anderson²

Affiliations

¹ Department of Pediatrics, School of Medicine, Emory University and Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA.
² Department of Pediatrics, School of Medicine, Emory University and Children's Healthcare of Atlanta, Atlanta, GA, 30322, USA. larry.anderson@emory.edu.

Abstract

Enzyme immunoassays (EIAs) to detect and quantify antibodies against respiratory syncytial virus (RSV) and RSV proteins in human plasma or sera are described. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The third EIA uses 30-amino acid synthetic peptides from central conserved region of G protein of RSV A2 or RSV B1 virus and a peptide from the SARS CoV nucleoprotein as a negative control peptide. All three EIAs have been evaluated for detecting and quantifying the respective antibodies in human sera or plasma.

Keywords: Enzyme immunoassay; Peptide; RSV F gene; RSV G gene; Respiratory syncytial virus.

MeSH terms

Antibodies, Viral / blood*
Antigens, Viral / immunology
Cell Line, Tumor / immunology
Cell Line, Tumor / virology
Humans
Immunoenzyme Techniques
Respiratory Syncytial Virus, Human / immunology*
Viral Proteins / immunology*

Substances

Antibodies, Viral
Antigens, Viral
Viral Proteins